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Avian cholera bacteria were found in 46 of 49 Adélie penguins sampled after a 2024 die-off

Researchers detected avian cholera bacteria in 46 of 49 Adélie penguins sampled after a 2024 die-off in Antarctica's Danger Islands. H5N1, the first suspect, turned up in only a few, so a test for flu alone would have named the wrong cause.

The Scientist · Science desk

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Illustration accompanying Avian cholera bacteria were found in 46 of 49 Adélie penguins sampled after a 2024 die-off
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An old, far-linked cholera strain hit a vast colony How long avian cholera has likely circulated in the region, how far away related strains were found, and the Danger Islands' Adélie breeding pairs.

Key figures: genetic analysis suggests avian cholera has circulated in the region for at least 20 years; close genetic links were found to strains more than 8,000 kilometers away in the Southern Ocean; the Danger Islands are home to more than 1.2 million Adélie penguin breeding pairs.

An old, far-linked cholera strain hit a vast colony
FigureValueNoteClaim
Years avian cholera has likely circulated, at least20 years7
Distance to related strains, more than8,000 kmin the Southern Ocean; 5,000 miles8
Adélie penguin breeding pairs, more than1,200,000 breeding pairson the Danger Islands3

What happened

  • An international expedition to the Danger Islands in March 2024 found thousands of dead Adélie penguins across two islands, plus carcasses of several other seabird species.
  • By the authors' reading, avian cholera has been present in the region for at least 20 years: the outbreak strain closely matched one identified in Antarctic seabirds more than two decades before.
  • The strain also has close genetic relatives among those detected more than 8,000 kilometers away in the Southern Ocean.

Why it matters

  • decision A die-off survey that tests only for H5N1 would have returned a few positives and the wrong cause, so bacterial testing and pathology belong in the same protocol.
  • constraint With avian cholera circulating for at least 20 years, the arrival of H5N1 on the Peninsula no longer dates or explains a mortality event on its own.
  • exposure Remote colonies that nobody samples cannot say which pathogen is at work, and Iervolino says outbreaks there can go undetected or uninvestigated.

Pasteurella multocida was detected in 46 of the 49 animals sampled [6], or 93.9% [14]. That leaves three animals without a detection [15]. Set against thousands of carcasses across two islands [2], 49 is a small sample, and the phys.org account of the study does not say how the animals were chosen or whether any carried both the bacterium and the virus.

The paper's claim is that most of the investigated deaths were caused by avian cholera [5]. Finding a bacterium in a carcass does not by itself show it killed the bird, and the team paired laboratory testing with pathological examinations [5]. In our view that pairing is what lets the authors speak of cause of death at all.

The genetics argue against a recent arrival. The outbreak strain was closely related to one identified in Antarctic seabirds more than two decades earlier, and the authors suggest avian cholera has circulated in the region for at least 20 years [7]. They also found close links to strains detected more than 8,000 kilometers away in the Southern Ocean [8]. H5N1 had only recently reached the Antarctic Peninsula [4]. At the moment of discovery, timing was the only thing connecting bird flu to the carcasses.

Ralph Vanstreels, a wildlife veterinarian at the UC Davis One Health Institute's Latin America Program and a senior author [11], put the caution plainly. "However, this case is an important reminder that other pathogens continue to circulate and cause mortality in these populations," he said [9].

One expedition in March 2024 [2], two islands and 49 animals show that avian cholera can drive a mass die-off in a colony of more than 1.2 million breeding pairs [3]. The study gives no rate across Antarctica. Lead author Matteo Iervolino of Erasmus University hedged accordingly. "The findings suggest avian cholera may be a more widespread and underestimated cause of seabird mortality in Antarctica than previously recognized," he said [10]. He added that sustained surveillance matters in remote ecosystems, where outbreaks can go undetected or uninvestigated [13].

The study is by Iervolino and colleagues in Proceedings of the Royal Society B, titled "Avian cholera causes mass mortality at penguin mega-colonies amid H5N1 HPAI outbreaks in Antarctica" [12].

What to watch

  • Whether follow-up work reports how the 49 animals were chosen and whether any carried both the bacterium and H5N1.
  • Whether surveys of other Antarctic colonies test for Pasteurella multocida alongside H5N1 after a mass death.
  • Whether further sequencing ties the Danger Islands strain to the Southern Ocean strains more than 8,000 kilometers away.

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  1. [1]

    Thousands of Adélie penguins found dead in Antarctica in 2024 were primarily victims of avian cholera, not high pathogenicity avian influenza (H5N1) as researchers initially suspected, according to a study in Proceedings of the Royal Society B.

    Reported Supported Source: phys.org report on Iervolino et al., Proceedings of the Royal Society B (2026)View cited source
  2. [2]

    In March 2024 an international research expedition to the Danger Islands found thousands of dead penguins across two islands, along with carcasses of several other seabird species.

    Reported Supported Source: phys.orgView cited source
  3. [3]

    The Danger Islands are home to more than 1.2 million breeding pairs of Adélie penguins.

    Reported Supported Source: phys.orgView cited source

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Sources

1 independent publisher whose own reporting we read for this story.

  1. phys.org

    1 article · October 14, 2026

    Scientists identify avian cholera as major cause of penguin deaths in Antarctica

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