Science1 publisher3 min readPublished
Two microbes in sequence more than triple the lysine in faba beans and rapeseed press cake
DTU and Aarhus researchers used two bacteria to raise lysine 3.4-fold in faba beans and 3.6-fold in rapeseed press cake. The main target is organic pig and poultry feed, where synthetic amino acids are barred and farmers feed extra protein instead.
The Scientist · Science desk

What happened
- Bacillus subtilis subsp. natto first breaks the plant proteins into smaller pieces, and Corynebacterium glutamicum then turns the released nitrogen into lysine.
- The study, published in the Journal of Agricultural and Food Chemistry, is part of NextOrganic, a project focused on animal feed.
- Christian Solem of DTU says methods to enrich crops with two further essential amino acids will be ready soon.
Compiled by The ScientistSomething wrong?How this is made
Why it matters
- capability If the gain carries through to animals, organic pig and poultry farms could meet lysine needs by changing an ingredient, without the supplement their rules forbid.
- constraint Until animal data exist, nutritionists can model the extra lysine in a ration on paper but cannot yet count on it when cutting total protein.
- decision Faba beans and rapeseed cake would bring more lysine into the comparison feed buyers already make between Northern European protein crops and imported soy.
The order in which the bacteria run is the core of the design. C. glutamicum makes the lysine, but only after the Bacillus step has cut the plant proteins down and released nitrogen it can use [2]. By that account, the new lysine is built from nitrogen the beans and press cake already held. Christian Solem of the DTU National Food Institute said the partial breakdown has a second use. "The interesting thing is that we can use microorganisms to improve the amino acid profile of plant-based ingredients while also making plant proteins easier to digest by partially breaking them down. This is particularly beneficial because many crops contain antinutrients that make it harder for our bodies to absorb the nutrients found in plant-based foods," he said [8].
Together, the fold changes and the final concentrations give the starting points. Faba beans began at about 11 g of lysine per kilogram of dry matter and rapeseed cake at about 8 g [1][2]. Fermentation therefore added roughly 26 g and 21 g per kilogram [3]. The fold figures are rounded to one decimal place, so those baselines are approximate.
The release says the result means more nutritional value from the same quantity of food or feed [13]. The measurement behind that statement is a concentration in dry matter after fermentation [4]. A per-kilogram figure can't tell you how much of the starting kilogram survived two rounds of microbial growth, and the release reports neither that nor any measurement of digestibility.
In feed, a shortfall of essential amino acids slows growth and can make animals ill. Producers deal with it today by feeding more protein than the animals need, or by adding amino acids produced through fermentation [9]. The DTU-Aarhus approach does the fermenting inside the ingredient itself [2]. Organic pig and poultry farms are not allowed the added route, according to Jan Værum Nørgaard of Aarhus University [10]. He said the surplus protein has a cost: "If the animals are given more protein than they can utilize, they excrete more nitrogen via urine and feces, which places a strain on the environment" [11].
In my view the organic feed case is the nearer application. The ban on added amino acids leaves a specific gap, and an ingredient that carries its own lysine is aimed straight at it. That view holds only if the dry-matter and digestibility figures come out well. Both crops are grown in large quantities in Denmark and the rest of Northern Europe, and both can replace imported soy in feed [12]. The researchers also say the method works well with other plant crops [14] and could help ease malnutrition in low-income countries [15]. The figures published so far cover faba beans and rapeseed press cake [4].
What to watch
- A pig or poultry feeding trial on the fermented faba beans or rapeseed cake that reports growth and nitrogen excretion.
- Whether organic feed rules treat lysine made inside the ingredient by C. glutamicum differently from added synthetic lysine.
- The processing cost of two fermentation steps at feed scale, set against the cost of simply feeding extra protein.